MOLECULAR DOCKING STUDIES OF ISOLATED COMPOUNDS ANDROGRAPHOLIDE AND BETULIN FROM METHANOLIC LEAVES EXTRACT OF ANDROGRAPHIS ECHIOIDES AS ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE ACTIVATORS

Objective: The purpose of this study is to isolate and characterize the andrographolide betulin from methanolic leaves extract Andrographis echioides also used evaluate alpha-amylase alpha-glucosidase inhibitory activity isolated compounds using in silico docking studies.
 Methods: isolation was done column chromatography gradient mobile phase. Structural elucidation carried out on basis spectral analysis. In view, were prepared for evaluation. studies a recent version Auto Dock 4.2, which has basic principle Lamarckian genetic algorithm.
 Results: On data, have been established as are being reported plant first time. result showed that binding affinity amylase: (-7.9 kcal/mol) glucosidase (-7.2 while (-8.6 (-5.2 kcal/mol), respectively.
 Conclusion: Therefore, it suggested contributed excellent α-amylase α-glucosidase because its structural parameters. Thus, these can be effectively drugs treating diabetes predicted scores.